Imashini Nshya yuzuye ya nucleic aside ikuramo kandi ikanamenya

Imbaraga eshanu:
● Igikoresho cyagizwe no gukuramo aside nucleic n'intambwe isukuye
● Igikoresho cyagizwe na ultrasonic yo gukuramo module
● Igikoresho cyagizwe naomatike yuzuye
● Igikoresho cyagizwe hamwe nubushyuhe bwo guhinduka
● Igikoresho cyagizwe hamwe na reagent yuzuye

1.Ese reagent ya nucleic acide nucleique igomba gukururwa kandi igakorwa?
Ihame ryo kumenya aside nucleique niyi ikurikira: mugikorwa cya primer, polymerase ya ADN ikoreshwa muguhindura urunigi rwerekana inyandikorugero ya ADN / RNA (bisaba ko hahindurwa transcript ya NA), hanyuma umubare wibimenyetso bya fluorescent wasohotse ukamenyekana kugirango umenye niba icyitegererezo kirimo aside nucleic (ADN / RNA) ya patogene igomba kumenyekana.

1) Ingero zidakuweho cyangwa ngo zisukure zishobora kuba zirimo ibintu byinshi bigira ingaruka kumusubizo wanyuma: nuclease (ishobora gushonga aside nucleic igamije kandi igatera ibibi bibi), protease (ishobora kugabanya polymerase ya ADN kandi igatera ibibi bibi), ibyuma biremereye umunyu (biganisha ku kudakora kwa synthase kandi bigatera ibyiza bitari byiza), acide cyane cyangwa alkaline PH cyane (ishobora gutera reaction kunanirwa), RNA ituzuye (biganisha ku kunanirwa kwandikirana nabi).

) ingero.

Niyo mpamvu, birasabwa guhitamo ibikoresho bipimisha cyangwa ibikoresho byahujwe nintambwe yo gukuramo aside nucleic.

2. Gukuramo imiti cyangwa gukuramo ultrasonic kumubiri?
Muri rusange, kuvoma imiti birashobora gukoreshwa mubice byinshi byo kwitegura na Puri fi cation.Nyamara, muri bagiteri zifite urukuta runini rwa Gram-positif na parasite zimwe na zimwe, ni nako bigenda bivamo imiti idashobora kubona urugero rwa acide nucleic acide ya e-ective, bigatuma habaho gutahura nabi.Byongeye kandi, kuvoma imiti akenshi ikoresha ibintu bikomeye, niba elution idasobanutse neza, biroroshye kwinjiza alkali ikomeye muri sisitemu yo kubyitwaramo, bikavamo ibisubizo bidahwitse.

Gutandukanya Ultrasonic ikoresha kumenagura umubiri, yakoreshejwe neza na GeneXpert, uruganda rukomeye muri fi yambere ya POCT kugirango ikoreshwe n'abantu, kandi ifite inyungu zuzuye mu gukuramo aside nucleique ikuramo ingero zimwe na zimwe (nka Mycobacterium tuberculose).

Niyo mpamvu, birasabwa guhitamo ibikoresho byo gupimisha cyangwa ibikoresho byahujwe nintambwe yo gukuramo aside nucleique.kandi nibyiza niba hari ultrasonic yo gukuramo module.

3. Igitabo, Semi-automatic kandi byikora byuzuye?
Iki nikibazo cyibiciro byakazi nakazi keza.Kugeza ubu, ibitaro byamatungo bidafite sta ff zihagije, no gukuramo aside nucleic no gutahura ni umurimo usaba ubuhanga nuburambe.Ntagushidikanya ko imashini ya nucleic yuzuye ikuramo kandi ikanamenya imashini ihitamo neza.

4. Ubushyuhe buhoraho ampli fi cation cyangwa ubushyuhe buhindagurika ampli fi cation?
Ampli fi cation reaction ni ihuriro ryerekana nucleic aside, kandi tekinoroji yumwuga igira uruhare muriyi miyoboro iragoye.Mu magambo make, enzymes zikoreshwa mukwongera aside nucleic.Mubikorwa bya ampli fi cation, ibimenyetso bya ampli fi ed fl uorescence cyangwa ibimenyetso byashyizwemo fl uorescence byamenyekanye.Muri rusange, ibimenyetso byambere fl uorescence bigaragara, niko intego ya gene yibintu byintangarugero.

Ubushyuhe buhoraho ampli ation cation ni acide nucleic aside ampli ation cation ku bushyuhe bwa fi xed, mugihe ubushyuhe bwimpinduka ampli fi cation ari cyclic ampli ation cation ukurikije cyane denatration-annealing-kwaguka.Igihe cy'ubushyuhe gihoraho ampli fi cation cyakozwe, mugihe ubushyuhe bwimpinduka ampli fi cation nigihe kinini cyane ff bigaragazwa nigipimo cyizamuka ryubushyuhe nigabanuka ryigikoresho (kuri ubu, ababikora benshi bashoboye gukora inzinguzingo 40 za ampliation muminota 30).

Niba laboratoire imeze neza kandi akarere gakaba gakomeye, birakwiye kuvuga ko itandukaniro riri hagati yibi byombi ritazaba rikomeye.Nyamara, ubushyuhe buhindagurika ampli ation cation izahuza ibicuruzwa byinshi bya acide nucleic mugihe gito ugereranije.Kuri laboratoire zidafite akarere gakomeye hamwe n’abakozi bashinzwe amahugurwa y’umwuga, ibyago byo gutembera kwa acide nucleic acide aerosol bizaba byinshi, ibyiza bitari byo bibaho iyo kumeneka bibaye, kandi bikaba bigoye gukuraho.

Byongeye kandi, ubushyuhe buhoraho ampli ation cation nayo ikunze kwibasirwa na ampli fi cation mugihe icyitegererezo kitoroshye (ubushyuhe bugereranije ni buke, kandi nubushyuhe bwo hejuru bwagutse, niko primer ihuza speci fi umujyi).

Kubijyanye nubuhanga bugezweho, impinduka zubushyuhe ampli fi cation ni iyo kwizerwa.

5. Nigute twakwirinda ibyago byo kumeneka kwa acide nucleic aside ampli fi cation?
Kugeza ubu, abayikora benshi bahitamo ubwoko bwa glande ubwoko bwa PCR nkumuyoboro wa nucleic aside reaction, ugafungwa no guterana amagambo, hamwe nubushyuhe bwubushyuhe mubihindagurika ryubushyuhe mubushyuhe butandukanye PCR ampli fi cation igera kuri dogere 90
Centigrade.Inzira isubirwamo yo kwaguka hamwe nubushyuhe no kugabanuka hamwe nubukonje ningorabahizi ikomeye yo gufunga umuyoboro wa PCR, kandi imiyoboro ya gland ya PCR iroroshye gutera kumeneka.

Nibyiza gufata reaction hamwe nibikoresho / bifunze neza kugirango wirinde kumeneka ibicuruzwa.Byaba byiza haramutse hashobora gukorwa ibikoresho bifunze byuzuye byo gukuramo aside nucleic no gutahura.

Imashini nshya ya Tech nshya ikuramo acide nucleic acide na mashini yo gutahura ifite amahitamo yavuzwe haruguru.