Mugihe cyo gusohora, poroteyine ifatirwa kuri BNP ikora physiologique ikora (acide ya 77 kugeza 108) na n-terminal igice cya NT-proBNP (acide ya 1 kugeza 76).Iyo BNP, ifite aside amine 32 z'uburebure, isohoka mu maraso, ihuza abayakira (NPRA na NPRB) kandi ikagenga umuvuduko w'amaraso binyuze mu buryo butandukanye.NT-proBNP, ifite aside amine 76 ndende, ntabwo ifite ibikorwa byibinyabuzima, ariko kubera ko ifite ubuzima burebure burenze icya BNP, irakwiriye cyane nkikimenyetso cyerekana indwara zitandukanye z'umutima.Mu isuzuma ry’amavuriro y’inyamanswa, amaraso ya NT-proBNP mu mbwa arenga 900 pmol / L, naho injangwe zirenga 270 pmol / L, kandi hari ibyago byinshi by’indwara ziterwa n'umutima.Icyakora, twakagombye kumenya ko kubera ko NT-proBNP isohoka nimpyiko, mugihe inyamanswa irwaye indwara zifata impyiko, kwibanda kwa NT-proBNP mumubiri nabyo biziyongera kandi ibyiza bibeshya bizagaragara mugupimisha.
Iki gicuruzwa gikoresha fluorescence immunochromatography kugirango umenye ibiri muri fNT-proBNP muri serumu / plasma mubwinshi.Ihame shingiro: Hano hari imirongo ya T na C kuri fibre fibre ya nitric aside, kandi umurongo wa T wasizwe na antibody a imenya neza fNT-proBNP.Ihuriro ryibikoresho byatewe hamwe na fluorescent nanomaterial yanditseho antibody b ishobora kumenya neza FDT-probNP.Muri sample, FDT-probNP yabanje guhuza na nanomaterial yanditseho antibody b kugirango ikore urwego, hanyuma kuri chromatografi yo hejuru, urwego ruhuza na T-umurongo wa antibody A kugirango ikore sandwich.Iyo umunezero wo kurasa urumuri, nanomaterial isohora ibimenyetso bya fluorescence.Imbaraga z'ikimenyetso zifitanye isano neza hamwe no kwibanda kwa fNT-proBNP muri sample.
Kuva yashingwa, uruganda rwacu rwateje imbere ibicuruzwa byambere byisi ku isi hubahirizwa ihame
y'ubwiza mbere.Ibicuruzwa byacu byamamaye cyane mu nganda no kwizerwa mu bakiriya bashya kandi bashaje ..